gene clone

基本解释

Acquiring barley mutants and research on them was key for gene clone and function analysis, and was the base of improvement for agricultural character using biotechnology.
大麦突变体的获得和深入研究是开展大麦基因克隆和功能研究的关键，是利用生物技术手段改良大麦农艺性状的基础。
The purpose of this paper is to review citrinin's physicochemical properties, toxicity, inhibiting bacteria activity, analytical methods, biosynthetic pathway, gene clone, etc.
本文介绍了红曲霉桔霉素的理化性质、毒性、抑菌性、检测方法、生物合成途径及基因克隆等内容。
The result showed that the PCR was very specific and sensitive to porcine circovirus and the virus gene clone, indicating that it is a good method for detection of porcine circovirus.
结果表明，PCR对猪圆环病毒细胞毒，病毒基因克隆具有较好的特异性和灵敏性，提示用PCR试验检测猪圆环病毒是一种较好的方法。

This is another way to clone or make many copies of a gene of interest.
这是另一种克隆出许多,你感兴趣的基因的方法
耶鲁公开课 - 生物医学工程探索课程节选
If I want to clone a gene from a human, if I want to clone the gene for human isulin, mean make many copies of the gene that's responsible for making insulin, I need to know whether there are introns there or not.
如果我想要从一个人类个体克隆基因,如果我想要克隆人类胰岛素基因,亦即我要把负责生成胰岛素的基因,复制许多个副本,我就得要知道哪些是内含子,哪些不是
耶鲁公开课 - 生物医学工程探索课程节选
How did we put our gene fragments into this plasmid DNA in order to make multiple copies of it, or to clone the gene?
如何把基因片段整合到质粒DNA上,来获得大量拷贝,或者说来克隆基因呢
耶鲁公开课 - 生物医学工程探索课程节选

Zeneca ordered a "wet" copy of a promising gene, in the form of a clone -- a colony of bacterial cells whose members all carry the desired snippet of DNA. These bacterial guinea pigs can then be used to try out antibacterial chemicals.
FORBES: Biology in silico

基因克隆
We constructed a recombinant strain by gene clone technology. The conditions of biosynthesis theanine were studied to improve output of theanine.
本论文通过基因克隆技术构建了工程菌,优化了工程菌催化合成茶氨酸的条件,从而达到提高茶氨酸产量的目的。
分子克隆
The results of the research demonstrated that the total RNA isolated by this protocol is of sufficient quality for gene expression and some other molecular biology research such as gene clone etc.
实验结果表明，此方法所得RNA质量足以满足基因表达和分子克隆等分子生物学研究需要。
生物学
基因克隆
Sequence analysis of 16S rRNA gene clone libraries showed that bacteria in G library at time point A were mainly Betaproteobacteria and Gammaproteobacteria,and also with low percentage of Defer-ribacteres,Firmicutes and Bacteroidetes;But in L library,97% bacteria belong to Moraxellaceae of Gam-maproteobacteria.
16S rRNA基因克隆文库结果表明,A时间点样品G中的优势菌群为Betaproteobacteria、Gammaproteobacteria,还有Deferribacteres、Firmicutes、Bacteroidetes等;而样品L中,Gammaproteobacteria中的Moraxellaceae含量达到97%。